An analysis is a method plus a batch of bottles. The method says what to look for and how to build the curve. The batch is the sequence you actually ran.

How to set up an analysis Five steps: method, samples, types and doses, analyze, review and report. Set up an analysis One method, one batch of bottles, then Analyze. Review and report use the same results. 1 Method Analytes, ISTD links Dose names C1&C5, IS AcqKeys / QntKeys Initial or Bracket 2 Add samples Batch -> Add samples Vendor data files One bottle = one file or sequence folder 3 Type + dose Blank / Cal / QC / Sample Cal bottles get C1&C5 ISTD uses dose IS Dilution if needed 4 Analyze Integrate Fit the curve Quantitate Flag outliers 5 Review Key Review Report Export Method -> Load / Import for a .qtm package or a vendor method. Save Method writes the current method, including calibrated responses. Sample types come from the method Sample types table (name match, then defaults). Override Type or Dose on the Samples grid when a bottle is special. Calibration mode lives on the method: Initial calibration = one curve. Bracketing = a curve per cal set.
Five steps: method, add samples, type and dose, analyze, review

Bottle types

Quanto has five analysis categories. Cap color in these drawings matches the method defaults (silver blank, coral calibration, blue QC, green sample, steel-blue solvent).

Bottle types in a Quanto analysis Blank, calibration, QC, and sample vials. Blue drops are target analyte. Pink drops are ISTD. Four bottle types Cap color follows the method sample type. Blue drops are target analyte. Pink drops are ISTD. Blank solvent only Calibration known level QC check recovery Sample unknown amount ISTD sample target + spike Target analyte — unknown in samples, known in calibration and QC ISTD — same known spike in every bottle that uses internal standard Blank — matrix or solvent. Used to confirm the target is absent. Calibration levels share dose names (C1, C2, C3&). Fill height in these drawings is the dose.
Blank, calibration, QC, sample, and ISTD sample bottles
Type What is in the bottle What Quanto does with it
Blank Solvent or matrix. Optional ISTD spike. Confirms the target is absent. Not a curve point by itself. With Origin = Blank offset, its response becomes the curve intercept — see Blank offset. With Blank corr, its on-curve concentration can be subtracted toward NativeConc — see Blank concentration.
Calibration Known target amount. Dose name C1, C2, … Builds the curve.
QC Known target amount, treated as a check. Read from the curve. Recovery / residual flags.
Sample Unknown amount. Read from the curve. Reported concentration.
Solvent Solvent vial — not a method blank. Read from the curve like Sample. Not used as a Blank use source. Blank-concentration subtraction is not applied to Solvent results (even when Blank corr is on). Blank offset can still apply through the shared curve.

Blue drops in the drawings are target analyte. Pink drops are ISTD. A bottle can hold several analytes; each analyte has its own curve.

Blank vs Solvent (short): use Type Blank for method / matrix blanks that should feed Blank offset or Blank corr. Use Type Solvent for a solvent check vial you only want to read from the curve — it will not subtract as a blank and will not supply blank bottles.

Build the method

Method → Load / Import

Then check these method tables:

Table Set this
Analytes Name, type (Target / ISTD / Surrogate), units, extraction window
Quants QntKey (ion / transition), curve fit, which ISTD Quant to use
Doses Shared names: C1…C5 for curve levels, reserved IS for the ISTD spike
Quant doses Which levels that Quant actually uses on the curve
Sample types How file names / metadata become Blank, Calibration, QC, Sample, or Solvent (there is no separate Spike / StdAdd sample type)
AcqKeys One set per imported method or instrument
CalGroup Optional label that groups bottles that share one calibration story (see CalGroup & standard addition)
Calibration mode Initial calibration (one curve) or Bracketing (a curve per cal set)
CalGroup Mode Per group: Calibration (normal curve) or StdAdd (standard addition)

A Quant with no ISTD link is ESTD. A Quant that points at an ISTD Quant is ISTD. You can mix both in one method.

Fortified amount = dose. When a Calibration, QC, or other known-level bottle has a dose, that known amount is the dose used for recovery (RecPct). You do not need a separate “spike” sample type — put the known amount on Dose.

Report workbooks: on the Samples grid you can multi-select which report workbooks run for each bottle (see Reports, import, and export).

Load the bottles

  1. Batch → Add samples and pick the data files (or a sequence folder).
  2. Each file becomes one sample. Type and dose are matched from the method, then from the file.
  3. Fix leftovers on the Samples grid: Type, Dose, Dilution, AcqKey, SmpKey.

Calibration bottles must have a dose name that exists on the analyte. Samples and blanks usually have no curve dose; ISTD bottles still use dose IS.

Analyze

Analyze integrates, fits each Quant’s curve, quantitates, and flags. After that:

If you use blanks: set Origin / Blank corr / Blank use on the Quants grid before Analyze. After Analyze, check Blanks used, Blank b or BConc, and Warnings on the result.

Which guide next

You are doing Read
One instrument, no ISTD, C1–C5 then unknowns ESTD initial calibration
ISTD spiked in every bottle ISTD initial calibration
Cal, samples, cal, samples Bracket calibration
Two instruments or several ions per analyte Key Review
Re-run only some levels in later batches Multi-batch dose responses
Spreadsheets, vendor files, Excel / PDF Reports, import, and export
Blank response on the curve Blank offset
Blank concentration toward NativeConc Blank concentration
Several curves / standard addition in one batch CalGroup & standard addition
Pass/fail and recovery flags Result limits & flags
Method Validation pane Method Validation

Grid gestures (fill, sort, freeze) are in Grids.