Limits tell Quanto when a result or curve point is outside what you accept. After Analyze, flags show up on results and in Method Validation.

Recovery on known doses

When a bottle has a known dose (Calibration, QC, and any other bottle you treat as known):

Field Formula / meaning
RecPct 100 × calculated concentration / dose
DevPct RecPct − 100
RecFlag Pass/fail against the recovery limits you set

There is no separate Spike sample type in the bottle list — put the known amount on Dose (for standard addition, use the spike increment). See Set up an analysis and CalGroup & standard addition.

ISTD and ESTD curves both produce concentrations that feed RecPct the same way once a dose is present.

Curve and response limits

Limit Typical use
MinCurveR2 Minimum acceptable coefficient of determination for the fit
MinRsp / MaxRsp Response (area or height) window for a peak
MinQValue Minimum qualifier / confirmation value
Qualifier counts How many qualifiers must pass
MinCoElution Minimum co-elution / overlap score when the method uses it
AboveCurveMaximum Flag when the result sits above the top calibrator
PassFailFlag Overall pass/fail roll-up for the result

Set these on the method / Quant tables before Analyze. Names match the columns you see in Quanto; leave a limit empty when you do not want that check.

Where flags appear

Practical tips

  1. Start with recovery limits on QC (and maybe Calibration) before tightening R² and response windows.
  2. After clearing a bad cal point (Use off), Analyze again so RecPct and curve flags refresh.
  3. Blank offset and blank concentration change concentration — re-check RecPct afterward (Blank offset, Blank concentration).
  4. For ISTD work, also watch ISTD response limits so a dead ISTD does not silently pass targets (ISTD).

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